Chronic toxicity of new pyridine-n-oxide derivatives for tetrahymena pyriformis w, determination of “structure-toxicity”, “concentration-time-effect” correlation

There is a considerable amount of work in the scientific literature on nonlinear (“paradoxical”) effects, but their toxicological significance for body still poorly understood. Plant growth regulators are biologically active substances at level low and ultra-low doses concentrations, they widely used agriculture. To date, mechanisms toxic specific effects body, presence “paradoxical” have not been sufficiently studied. Thus, it important to further indepth study “concentration-time-effect” relationship under chronic exposure which will be understanding general biological processes, developing approaches hygienic regulation risk assessment human health non-target environmental objects. The Aim Research. Determine dependence “structure-toxicity”, plant – pyridine-N-oxide derivatives conditions ciliates Tetrahymena pyriformis W. Materials Methods. object research was complexes methyl with organic acids metal salts. Studies were performed W stationary phase growth. effect studied 96-hour experiment concentration range from 1×10 -2 M 1×10-28 M. initial number sample 20,000 cells/ml culture medium. samples incubated dry air thermostat 25°C. After 24, 48, 72, 96 hours, different phases determined. experiments four replicates. results subjected mathematical processing by methods variation statistics using standard software package Microsoft® Office Excel 2010. Results. It shown that 1×10-2 investigated N-oxidepyridine salts more than acids. In concentrations 1×10-4 "concentration-timeeffect" detected. Regardless duration action, both inhibition induction ciliate population observed. change direction observed high, ultralow concentrations. Conclusions. 1. Under W, rate M, DG-377 (Di-2,6-dimethylpyridine-N-oxide ZnCl2), DG-380 ZnI2), DG-387 CoCl2), DG-480 (2-methylpyridineN-oxide DG-471 (Di-2-methylpyridine-N-oxide CoI2) similar DG-361a (2,6-dimethylpyridine-N-oxide succinic acid), DG-361 (Di-2,6-dimethylpyridine-N- DG-362 maleic DG-349a (2-methylpyridine-N-oxide acid). 2. -28 regardless time there population. population, depending exposure, polymodal nature. 3. According severity DG-361a, DG-361, DG-377, DG-387, characterized mainly an inhibitory effect. stimulating some moderate or weak. For DG-362, pronounced stimulation ciliates. Key Words: pyridine-N-oxide, ciliates, “concentration-time-effect”.